Attenuative Effect of Zingerone on Behavioural Anomalies and Oxidative Stress in The Cerebellum of Prenatal Valproic Acid Induced Autism Spectrum Disorder Like Symptoms in F1 Female Pups: In Vivo Modelling (Research Article)

Author(s): Sumathi Thangarajan* and Shruthi Sampat Kumar

Abstract: The pilot study aimed to evaluate the attenuative effect of zingerone (ZNO) against valproic acid (VPA)-induced autism spectrum disorder (ASD) like symptoms in the cerebellum of female rat pups. Pregnant female rats were administered with VPA (500mg/Kg/i.p) as a single dose on the 12th day of gestation to induce autistic phenotypes. The effect of ZNO at 30mg/Kg/p.o and 60mg/Kg/p.o on VPA-induced neurobehavioural anomalies (open field test, rotarod, forced swim test, and y-maze), oxidative stress (lipid peroxidation (LPO), total nitric oxide (NO)), altered antioxidative status (reduced glutathione (GSH), vitamin C (Vit-C) and catalase (CAT)), and histopathological staining (H&E staining) were assessed. Autistic behavioural anomalies were substantially diminished in a dose-dependent manner following ZNO administration. VPA-exposed female pups had elevated levels of LPO and total NO, which were significantly attenuated by ZNO. Treatment with ZNO also substantially promoted the levels of GSH, Vit-C, and CAT in the cerebellum. In the present study, for the first time, we report that ZNO showed promising results in attenuating VPA-induced symptoms, indicating a possible neuroprotective effect against the core symptoms of autistic female F1 rat pups.

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