Green Synthesis of CuNPs as Bio-Controlling Agents Against Sclerotium rolfsii Causing Stem Rot sickness of Arachis hypogaea L. (Research Article)

Author(s): P. Sreenivasa Murthy, Nookala Supraja* and M Nagalakshmi Devamma*

Abstract: Groundnut stem rot caused by Sclerotium rolfsii Sacc causing stem rot on more than 500 plant species of agricultural and horticultural crops throughout the world. Most of the first symptom associated with S. rolfsii are usually yellowing and wilting of leaves following stem rot infections. Biological management of the disease through antagonists is an eco-friendly approach apart from better alternative to the use of chemicals. In the present study, the thirty antagonistic microorganisms were evaluated by dual culture technique for their antagonistic effect against S. rolfsii under in-vitro conditions. Maximum inhibition of mycelial growth (92.78%) was noticed in Hypocrea koningii (Gunthakal field isolate) which was followed by T. Viride (Kadiri field) (63.33%). Least inhibition was observed in T. harzianum RDGFI isolate (31.67%). The results indicated that the application of these micro-organisms successfully decreases the stem rot incidence and also increases the growth of the groundnut plants. As the Hypocrea koningii has several benefits to crop plants. The efficient usage of antagonist was needful. To utilize the antagonist effectively copper Nanoparticles are biosynthesized by reducing copper nitrate. The size and surface Plasmon of Cu NP are compatible to enhance the efficacy of antagonist. However, CuNPs talc-based powder formulation was developed and commercialized. For this These Hypocrea koningii copper Nanoparticles were characterized by using the techniques such as X-ray Diffractometry (XRD), Fourier transform infrared spectrophotometry (FT-IR), UV–Vis spectrophotometry, Dynamic Light Scattering (Particle size), Zeta Potential and Transmission Electron Microscopy (TEM) with EDX.

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