Evaluation of Anxiolytic Activity of Hygrophila auriculata Seeds on Experimental Animals (Research Article)

Author(s): A. Katariya*, V. Gajera, V. Lambole, D. Shah and H. Joshi

Abstract: To study the anxiolytic activity of ethanolic and aqueous extracts of Hygrophila auriculata seeds in experimental animals. Hygrophila auriculata seeds powder extracted in two different solvents, one is ethanol and other is aqueous. standardization of both extracts and identification of phytochemical analysis test. The extracts were orally administered at the dose of 300-600 mg/kg of body weight. Elevated plus Maze, Light-Dark model, Open Field test for studying the anxiolytic behaviour and the Rotarod was used to check the motor co-ordination and Photoactometer used for locomotor activity. Both extracts showed significantly increased the percentage of time spent and number of entries in open arm in Elevated plus maze model. In Light-Dark model, the extracts produced significant increase in time spent in Light area. In Open Field test, the extract showed significant increase in number of squares crossed, all of which are demonstrations of exploratory behaviour. In Rotarod model showed significant decrease in the motor co-ordination score with fall of time of the mice from the rotating rod which indicates its anxiolytic-like effect. In locomotor activity evaluated with photoactometer in lower cut off number point out from extracts treated groups as per diazepam treated standard group. Both extracts showed prominent anxiolytic activity, but the best result obtained with 600 mg/kg dose of ethanolic extract of Hygrophila auriculata in models. The present research suggests that Hygrophila auriculata may be a therapeutic candidate for the treatment of Anxiety disorder.

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