Development and Validation of Rapid RP- HPLC Method for Estimation of Ondansetron in the Tablet Dosage Form (Research Article)

Author(s): R N Varade*, P Tak and H Mishra

Abstract: The developed reverse phase high-performance liquid chromatographic (RP-HPLC) method for the analysis of Ondansetron tablet dosage form is precise and feasible. The separation was carried out on a Fortis C18 (4.6 x 100 mm, 2.5 μm) column, using Methanol: 0.1 % OPA (Ortho-phosphoric acid) in ratio of 50: 50 % v/v as the mobile phase with flow rate at 0.7 mL/min and analysis was performed at wavelength 248 nm at ambient temperature. The injection volume was 20 μL. The retention time of the drug was 4.77 min. The method produced linear responses in the concentration range of 10 to 50 μg/mL. The LOD and LOQ values for HPLC method were found to be 0.11 and 0.32 μg/mL respectively. The method was validated as per ICH norms. The use of short column made method consumable. The method is also cost effective. The proposed method is useful for rapid analysis of Ondansetron in pharmaceutical dosage forms.

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