Evaluation of Cytotoxic, Antimicrobial and Antioxidant Activities of Phyto-synthesized Silver Nanoparticles of Tephrosia calophylla Bedd.

Author(s): Gorantla Ramakrishna and *Nimmanapalli Yasodamma

Abstract: The plant mediated synthesis of nanoparticles has significant application in biomedicine due to its novel properties and its eco-friendly nature. The present study deals with the biosynthesis of stable silver nanoparticles (SNPs) from aqueous rhizome extract of Tephrosia calophylla (TC). The synthesized nanoparticles are characterized by UV–Vis spectroscopy, Zeta potential, FTIR, XRD, TEM and EDAX methods. Colour change of the synthesized solution observed from Gray to brown indicates the formation of nanoparticles and UV–Vis surface plasmonresonance spectroscopy observed at 435 nm further confirmed the synthesis of nanoparticles as SNPs. FTIR spectroscopic studies confirm that phenols and proteins of rhizome extracts are mainly responsible for capping and stabilization of synthesized SNPs. The XRD data shows crystalline nature of nanoparticles and EDAX measurements reveals the presence of 69.56% Ag metal. Zeta potential at -19.9mV, negative value indicates the high stability of Nanoparticles. TEM microscopic analysis revealed that the size of synthesized SNPs ranges from 7 to 32.4 nm with spherical shape. Antimicrobial studies of AgNPs showed highest Zone of Inhibition against Staphylococcus aureus (32.75mm) among bacterial strains. Antioxidant activity of AgNPs shows 59.56%. Potential anticancer activity towards MDA MB 453 (human breast cancer cell line) cancer cell lines, shows 82.54% cell death with cell viability 17.46%. Overall, T. Calophylla is efficient in the synthesis of nanoparticles paves a way for better antimicrobial and anticancer therapeutic drug to be design by pharmaceuticals.

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