A Pre-Clinical Study of Glycyrrhiza Glabra Linn. For Treatment of Male Sexual Disorders Using Pharmacognostical, Chromatography (HPTLC and HPLC) and Spectroscopy (UV-Visible and FTIR) Chemical Analysis (Research Article)

Author(s): Mradu Gupta* and A. K. Mondal

Abstract: An Ayurvedic formulation prepared from dried root powder of Glycyrrhiza glabra Linn. is standardized through pharmacognostical and phytochemical pre-clinical studies for treatment of male sexual disorders. While total ash value was 6.27%, acid insoluble ash was 0.17% and water soluble ash was 4.1%. Among elements, Nitrogen was found present. Flavonoids, carbohydrates, glycosides, tannin and saponin were found present in both alcoholic and aqueous extracts. The results also showed high concentrations of flavonoidic compounds (27.40 μg Quercetin equivalent / mg) and phenolic content (29.50 μg Gallic acid equivalent / mg) in alcoholic as compared to aqueous extract. HPTLC analysis at 280 and 360 nm indicated the presence of Ellagic acid in both these extracts. Similarly, HPLC analysis at 276 nm showed elution of five compounds in aqueous and ten compounds in alcoholic extract whose analysis confirmed the presence of Ellagic acid in both extracts. UV-Visible spectroscopy scanning showed peaks at 315, 265.5 and 194.5 nm in aqueous and at 896, 315, 271 and 211 nm in alcoholic extract. FTIR analysis indicated presence of C-H stretching, Aryl -CH3 in-phase bending, -CH3 in-phase bending, Aryl out-phase stretching and Aryl 5 adjacent C-H wagging functional groups in aqueous and (N)-CH3 in-phase bending, Tertiary butyl-CH3 in-phase bending, (S)-CH3 in-phase bending, (S)-CH3 in-phase bending and -NO2 in-phase stretching functional groups in alcoholic extract suggesting the presence of phenolic & alkaloidal group of compounds in the extracts. The presence of phenolic and flavonidic compounds in research formulation extracts may be responsible for its antioxidant, analgesic, antipyretic, anti-inflammatory and antimicrobial properties.

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