Volume 10, Issue 3, July 2020

High-Performance Thin-Layer Chromatographic (HPTLC) Analysis of Roots of Euphorbia hirta Linn. (Research Article)

Author(s): Rashmi* and Pankaj Gupta
Abstract: India is well known for its Ayurveda medicines worldwide. In India it is estimated that about 8000 plants are used in traditional and herbal medicine. There are many species of Euphorbia from family Euphorbiaceae which are used in traditional medicines. The aim of the present study was to check the presence of phytochemical constituent flavanoid, using HPTLC analysis. The root extract of Euphorbia hirta was prepared using solvent alcohol. The extract has been subjected to HPTLC analysis for flavanoid profile. Quercetin was used as standard. The presence of flavanoid in alcoholic extract was confirmed based on the colour zones obtained. Quercetin was isolated from the diethyl ether fraction of alcoholic extract by solvent - solvent extraction technique and was identified and characterized by chemical tests, melting point and by a simple, sensitive, and validated HPTLC method. So, the present study explores the presence of flavonoid (quercetin) in the roots of Euphorbia hirta and future work aimed to isolate the flavonoid in the plant.
PAGES: 161-165  |  25 VIEWS  35 DOWNLOADS

How To Cite this Article:

Rashmi* and Pankaj Gupta. High-Performance Thin-Layer Chromatographic (HPTLC) Analysis of Roots of Euphorbia hirta Linn. (Research Article). 2020; 10(3): 161-165.