Comparative In vitro Evaluation of Anti- Diabetic Activity of Callus and Bulb Extract of Allium sativum L. (Research Article)

Author(s): Babita Rana* and Rinku Patole

Abstract: In the present research, comparative in vitro study has been made to evaluate the anti-diabetic potential of callus and bulb extract of Allium sativum L. Callus was induced from bulb explants in MS medium supplemented with 2, 4-D @ 0.5mg/l. sufficient amount of callus was obtained after 4 weeks of inoculation of explant. Callus was maintained by sub-culturing to have continuous supply throughout the project work. The extracts of callus and garlic bulb prepared in methanol were evaluated for their anti-diabetic activity by α-amylase and α-glucosidase inhibitory activities and were well compared with standard Acarbose drug. It could be concluded that due to the presence of anti-diabetic components the callus and plant extract have well prospective for the management of hyperglycemia. This knowledge will be helpful in isolating, characterization and purification of more potent anti-diabetic principle from callus culture to the clinical development of anti-diabetic therapeutics.

PAGES: 32-36  |  34 VIEWS  49 DOWNLOADS