Volume 10, Issue 2, April 2020

Isolation and Characterization of Lipase Producing Bacillus Sonorensis and Bacillus Halotolerans from Oil Contaminated Soil of North Telangana (Research Article)

Author(s): Ramya chouhan, Nagaraju Alupula, Suresh Lapaka and Srinivas Podeti*
Abstract: In the present observation, isolation and primary screening were conducted for the lipolytic activity of various bacterial species with reference to different oil-contaminated soils. The soil samples were collected from oil-contaminated effluent in Warangal region, North Telangana, India. The soil samples were analyzed for the presence of lipolytic bacterial strains and screened on Tributyrin agar plates for detection of lipolytic activity. Out of 70 bacterial colonies, 40 isolates were found to be lipase positive. Of the isolates, Bacillus sonorensis had shown maximum lipase production activity, whereas the B.holotolerans showed minimum activity. During the screening process of the bacterial strains, the existence of different predominately rod-shaped and cocci (SP5 and SP9) species were noted. Even though positive results for starch hydrolysis was observed, whereas the oxidase test was found negative for SP4, SP5, and SP9 and other samples. The SD2 strain had shown the highest lipolytic activity (4.75 m/ml) after 48 hrs. of incubation, whereas SD1 strain had shown lowest lipolytic activity (3.95 m/ml) followed by SB2 and SH3. Molecular identification was carried out based on 16s rDNA sequences and a phylogenetic tree was constructed to specifically identify the species. We were successful in identifying the two bacterial strains, namely, Bacillus sonorensis RCPS1, and Bacillus halotolerans strain RCPS2, responsible for lipase production from oil effluent contaminated soils of Warangal.
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How To Cite this Article:

Ramya chouhan, Nagaraju Alupula, Suresh Lapaka and Srinivas Podeti*. Isolation and Characterization of Lipase Producing Bacillus Sonorensis and Bacillus Halotolerans from Oil Contaminated Soil of North Telangana (Research Article). 2020; 10(2): 1-16.