Isolation and Characterization of Xylanase Producing Bacteria and Optimization of Parameters for Xylanase Production

Author(s): Sagalgile R.M, Khanna Simran S, Pandey Alok R and Vemula A.N.

Abstract: A soil sample was collected from wood industry & Mangroove soil from Vasai creek (Maharashtra) for microbial and enzymatic study. Xylanase producing bacterial strain were isolated and after further screening, three bacterial strain were selected from the soil sample. All they are gram positive in nature. These three bacterial strains were screened as xylanase producers. The bacterial isolates were examined for Morphological, Physiological and Biochemical characteristics along with optimization of temperature and PH for improved xylanase activity. The Bacterial isolates were having potential to produce the xylanase enzyme were analyzed by enzyme assay. 62.39 IU/ml was the maximum enzyme activity found. The Purified fractions of enzymes were used to clarify the fruit juice, which revealed that in 6 hrs 46% clarification of juice can be obtained.

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