Evaluation of Antiulcer Activity of Ethanolic Peel Extract of Punica granatum in Rats

Author(s): Syeda Husna Fatima, E. Madan Mohan and Syeda Hafsa Mehjabeen

Abstract: The present work incorporates the study of gastric antiulcer of dried Punica granatum (PG) peel 50% ethanol extract (PGE) in rats. PGE (200 mg/kg & 400 mg kg) was administered orally once daily to rats either before or after induction of gastric ulcers (GU) for 7 d. Antiulcer effects of PGE were seen against acute GU, induced by Pylorus Ligation (PL), Indomethacin induced, Cold Restraint Stress (CRS) in rats. Ulcer index (UI), Gastric juice volume, pH of gastric HCl, Free acidity and Total acidity were estimated. PGE showed a decrease in UI in PL method (23.77% to 36.42%) indicating both protective and healing effects. PGE showed effects on volume of gastric HCl (3.95+0.44**), pH of gastric acid (3.21+0.29**), Free acidity (59.16+5.32**), Total acidity (86.66+7.03**) in PL rats. In NSAIDs induced and cold restraint stress induced gastric ulcer models, 50% Ethanolic extract produced significant dose dependent decrease in ulcer index (34.68%) and (37.07%),P<0.01**, and it was found that maximum ulcer protective activity was shown by Ethanolic extract when given orally at a dose of 400 mg/kg body weight. Stat istical analysis was carried out by one-way analysis of variance followed by Dunnet’s test. The effect of Anti-ulcer activity of Punica granatum seems to be dose dependent and significant. The presence of Flavonoids which have astringent property could be responsible for the Anti-ulcer activity, is more likely to be involved in the reaction with the proteins of the layer tissues and thereby showing the activity.

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