A Brief Review on Recent Advances in HPLC Techniques

Author(s): Kharat Sachin Sadanand*, Andhale Saurabh Prakash and Saudagar Ravindranath Bhanudas

Abstract: In the present time chromatography has proven to be a significant technique and it is conventionally used for analysis purpose. High-performance liquid chromatography (HPLC) is a principal technique of current pharmaceutical and biomedical analysis to separate molecules from heterogeneous solutions and for further characterization preferably used preceding to mass spectrometry in the identification of peptides, proteins and other molecules. HPLC grew popularity due to its reliability (usage of pressure driven liquid support) and versatility (capacity of maintaining the composition of both mobile and stationary phases). Though fast separation often results in very high operational pressure, which places a huge load on HPLC instrumentation. Owing to which in recent years, core-shell silica microspheres (with a solid core and a porous shell, also acknowledged as fused-core or superficially porous microspheres) and Zirconia packing’s have been discovered. Designed for highly proficient and fast separation with reasonably low pressure for separation of small molecules, large molecules and complex samples. Ultra-high performance liquid chromatography (UPLC) can provide greater resolution, increased sensitivity and faster separation times compared with traditional HPLC, but sometimes it can also be less reproducible. The Rapid Resolution Liquid chromatography (RRLC) method is not only accurate but also sensitive; it effectively increases the sample analysis quality and quantity compared with conventional HPLC. The aim of this review is to highlight fundamental aspects on column technology and developments for Nano- bore, Micro- bore, RRLC, UPLC, UFLC, Nano LC. Also to focus on fundamentals of HPLC.

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