Isolation and Screening of Lipase Enzyme from Bacterial Strains Isolated from Sambhar Lake

Author(s): Ritika Joshi and Tara Singh Bisht*

Abstract: Sambhar Salt Lake Rajasthan (26°58′N 75°05′E) is a unique solar saltern and is a heaven for halophiles that belong to the extremophiles group. The potential of using bacteria as source of industrially important and relevant enzymes has stimulated interest in exploration of enzymatic activities. Lipase is one of the most important naturally occurring polymers and appears to be the cheapest future raw material of detergent industry. The current study was taken up mainly to study moderate halophiles of the lake. The halophilic bacteria are able to survive on 5 to 15% NaCl. This paper proposes to discuss some recent work on the isolation and screening of lipase enzyme. Bacterial strains were selected for screening onto tributyrin agar plates and clear zones around the colonies indicated the production of extracellular lipase and p-Npp estimation was done to confirm lipase production.

PAGES: 1324-1329  |  27 VIEWS  46 DOWNLOADS