Production of Secondary Metabolite (Lovastatin) From Edible Mushrooms Agaricus bisporus and Calocybe indica

Author(s): S. Yuvashree and C. Soundhari*

Abstract: Aim: In the present study an attempt was made to isolate Lovastatin from two different species of mushrooms such as Agaricus bisporus (white button mushroom) and Calocybe indica (white milky mushroom) and to estimate its potential toxic effect against HeLa by MTT assay. Methods: The Fruiting body of Agaricus bisporus and Calocybe indica were dried, powdered and extracted with ethyl acetate at 28 C for 24 hours and estimated for the quantitative analysis of lovastatin through the UV-spectrophotometer at different nanometer from 230-260. The isolated lovastatin was subjected to HPLC analysis for the confirmation. Results: The maximum absorbance was obtained at 240 nm for Agaricus bisporus with the absorbance OD value 3.4618 and also at 240 nm for Calocybe indica with the absorbance OD value 3.509. Lovastatin samples showed similar peak area ranges and retention time was 2.148 for A.bisporus and for C. indica the retention time was at 2.527 which indicates the presence of lovastatin in the extracted samples. Cytotoxicity is one of the properties of anticancer agents. The effective concentration of 50% cell death was found to be at 31.2μg/ml or the extracts of A.bisporus and 62.5μg/ml C. indica respectively on human cervical cancer cell lines (HeLa cell lines). Conclusion: The presence of lovastatin a cholesterol lowering agent has been confirmed in A.bisporus and C. indica spectroscopic and chromatographic analysis. Hence these mushrooms can be used as a functional food with cholesterol lowering effects.

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