Isolation and Characterization of Multi Drug Resistant Escherichia coli Isolated from Urinary Tract Infected Patients

Author(s): Mukesh Kumar and Jaya Bharti

Abstract: Incessant uses of antibiotics cause the antibiotic resistance, enhance special pattern of antibiotic resistance among the E. coli isolates which are obtain from patient surfing with urinary tract infection (UTI). This study was conducted to spot and isolate resistant multi drug uropathogenic Escherichia coli from piddle sample of tract infected patients by ancient techniques. Fifty-seven (57) UTI piddle samples were collected from patients admitted to near ELITE DIAGNOSTIC CENTRE BHILWARA (RAJ). Out of fifty-seven piddle samples, forty samples square measure positive for E. coli among that twenty-two samples square measure collected from females and eighteen from male. Growth for E. coli confirmed by culture characteristics (Table five.1) and then different biochemical test (Table five.2). Characterization of isolated strains resolve by biochemical tests included indole, oxidase, catalase, methyl red, voges proskauer, citrate utilization, hemolytic, motility and microscopic finding done by Gram staining. Sensitivity pattern of isolates were determined against antibiotics. From this study, it had been discovered that 70.17% isolates were gram negative. The E. coli positive samples square measure shows indole, enzyme (+ve) and VP, PYR, OXIDASE, UREASE, citrate Utilization (-VE) (table five.2). Positive samples square measure any analyzed for antibiotic susceptibleness testing among 40 samples 33 square measure showing resistance for quite 15 antibiotics. 2 samples were resistance for quite 20 antibiotics. different sample square measure resistance for concerning 5 to 10 antibiotics table (5.3, a, b, c). Out of 57 sample 40 uropathogenic E. coli was isolated and every one of them has been multi drug resistant.

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