28 Days Repeated Dose Oral Toxicity Study of Hydro-Alcoholic Extract of Celosea argentea Seed in Wistar Rats

Author(s): Yadav Nishigandha Subhash*, Kolhe Swati and Tembhurne Sachin

Abstract: The aim of this study was to evaluate the subacute oral toxicity study of hydroalcoholic extract of Celosea argentea seed (HAECAS) in male and female wistar rats. 28 days oral toxicity study of Celosea argentea seed extract was performed at doses of 200, 500 and 1000mg/kg. During the study period all the animals were observed for weekly body weight, food consumption, behavior changes, any sign of morbidity and mortality. At the end of study, the animals were humanely sacrificed and assessed for biochemical, hematological analysis and histopathological examination. The results of the study indicated to increase in the body weight and food consumption at 500 and 1000 mg/kg. The results of biochemical and hematological examination revealed significant alteration in the MCH, neutrophils count, lymphocytes, albumin, total protein and creatinine level at higher dose of HAECAS. In the histopathology, animals treated with extract at 500 and 1000mg/kg body weight showed minimal to mild hepatocellular vacuolation in liver and tubular degeneration at high dose in kidney; however, heart and lungs did not show any toxicity when compared with control group animals. From results, it concludes that repeated administration of hydroalcoholic extract of C. argentea increases the body weight as well as food consumption indicates its role as dietary supplement. The hydroalcoholic extract of C. argentea was found to be safe in 28 days repeated dose toxicity study. While at higher dose i.e. 1000 mg/kg there was increased in neutrophil count, creatinine, albumin, total protein and decreases the lymphocytes count. There was also mild to moderate congestion, and tubular degeneration at higher dose of HAECAS.

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