Extraction of Neem (Azadirachta indica) Seed Oil and Its Applications

Author(s): Amar Gaje, Jaishree Dange, Pavan Golwal, Shubham Tathe and Kalyani Pawar*

Abstract: Neem seed kernel represents the most important source of active ingredients found in neem tree, such as properties of extracted neem oil was also determined. In this experiment, Neem oil is extracted from kernels of Neem seeds. A soxhlet extraction was used to extract the oil. The extract was concentrated by using rotary evaporator. Results revealed that the yield of neem seed oil by using soxhlet extraction is 28 %. E. coli was the predominant isolate isolated from the urine specimen followed by Klebsiella sp., S. aureus, Pseudomonas Sp. The antibacterial activity of neem oil was evaluated against the 4 urine clinical isolates by broth dilution method and agar well diffusion technique, and zone of inhibition was measured in mm. In our study, highest antibacterial activity was recorded in S. aureus, Pseudomonas Sp. Insecticidal activities of neem oil have been studied against gram pod borer and spider mites. Oil extracted from neem seed kernels showed greater lethal properties on the insects.

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