Stability Indicating Chromatographic Method for Estimation of Methimazole

Author(s): Damle M. C.* and Pardeshi K. K.

Abstract: A stability indicating high performance thin layer chromatography (HPTLC) method was developed and validated for determination of anti-thyroid drug, Methimazole. HPTLC separation was carried out on Merck TLC aluminium sheets precoated with silica gel 60F254 using mobile phase as Chloroform: Acetone. Methimazole gave sharp peak at Rf 0.44 ±0.03 at 252nm. Calibration curve was linear in range 200-600ng/band for methimazole. Stress degradation study was carried out according to ICH guidelines Q1A (R2) and the method was validated as per ICH guideline.

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