RP-HPLC Analytical Method Development and Validation of Metformin Hydrochloride Tablets Assay

Author(s): Pavan Kumar Chadalawada*, Velupla Divya, Challa Adilakshmi, Puvvala Sreevidya and Imran khan. Ch

Abstract: An easy, definite and sensitive RP-HPLC method assay was developed and validated for Metformin Hydrochloride in pure and tablet dosage forms. The method uses Hypersil ODS C18, 25cm x 4.6mm x 5μm column and isocratic elution. Mobile phase was by adding 65% acetonitrile and 35% phosphate buffer and PH was adjusted to 5.75 with 85v/v Ortho phosphoric acid was used at a flow rate of 1.0 ml/min. The UV detection was programmed at 233 nm. All statistical values are within the acceptable range and parameters are validated by statistical methods. The new method of development was efficient for quantitate estimation of the pure and Metformin Hydrochloride tablet.

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