Screening of Actinomycetes for Lipase Inhibitors Production

Author(s): Chandwad S. C and S. L. Gutte.

Abstract: Obesity has become a serious health problem; it leads to diabetes, cardiovascular & musculoskeletal disorders and some types of cancer. One of the approach for treatment and control of obesity have involved inhibition of dietary lipid digestion by Pancreatic Lipase (PL) inhibitors. Products of natural source provide a vast pool of enzyme inhibitors including pancreatic lipase inhibitors that can be developed antiobesity drug. Actinomycetes are potential sources of enzyme inhibitors, drugs, amino acids, vitamins etc. Present work mainly highlights on the screening of actinomycetes extracts for PL inhibitors. Isolated 110 actinomycetes strains grown in fermentation condition and metabolites are extracted, extract of isolates tested for enzymatic inhibition. 14 extracts have shown positive results for enzyme inhibition and reconfirmed inhibitory activity using enzymatic assay by spectroscopic method. 10 extracts were shown PL inhibitory activity ranging from 10-80%. It concludes actinomycetes are potential source for PL inhibitors, which may lead to valuable novel drugs for obesity treatment.

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