Thrombolytic Activity of Syzygium cumini Seed Extract: An In-Vitro Evaluation

Author(s): AM Barbhuiya* And R Godiya

Abstract: The aim of the present study was to evaluate the thrombolytic potential of the ethanolic seed extract of Syzygium cumini. Sterile microcentrifuge tubes were weighed and labeled properly. 2.5 ml of fresh blood was drawn from each human volunteer and 500μl blood freshly collected blood was then transferred to separate previously weighed microcentrifuge tubes. They were then kept to form clot. Each properly labeled blood filled microcentrifuge tube was then incubated at 370C for 45 minutes. After clot formation, serum was withdrawn completely, without disturbing the clot. After that each tube was weighed again to get the weight of clot. Clot weight = weight of clot filled tube – weight of empty tube. To each microcentrifuge tube containing clot, 100μg/ml, 250μg/ml of ethanolic seed extract of Syzygium cumini (100μl) were added. In the control, 100μl of distilled water was added and in the standard 100μl of Streptokinase was added. All the tubes were then incubated at 370C for 90 minutes and observed for clot lysis. After incubation, supernatant fluid released was removed and tubes were again weighed to observe the difference in weight after clot disruption. Difference between previous weight and at now was noted to obtain the percent of clot lysis. The ethanolic seed extract (250μg/ml) of Syzygium cumini exerted 34% clot lysis from clotted blood in thrombolytic activity test while for standard (streptokinase) and control are 79% and 3% respectively. The findings of the present study revealed that the ethanolic seed extract of Syzygium cumini possess moderate thrombolytic activity.

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