Isolation, Characterization and Molecular Identification of Endophytic Bacteria from Mangrove Plants

Author(s): L. Elizabeth Jacquline, K. Kuzhalvaymani, T. S. Subha*

Abstract: Mangroves are plants that grow along coastal saline or brackish water, they occur worldwide in the tropics and subtropics, mainly between latitudes 25˚S. Microbes that live symbiotically within the plants are called endophytes. The aim of this study is to confirm the ability of endophytic bacterias in production of extracellular enzymes of economic importance. In the present study, 44 strains of endophytic bacteria were isolated from mangrove plants viz. Rhizophora annamalaiana, Rhizophora appiculata, Suaedo monoica, Ceriops decandra, Rhizophora mucarneta. The isolated bacterial strains were tested for various extracellular enzyme of industrial importance viz. Amylase, Gelatinase, Cellulase, Protease, Catalase, Pectinase, Chitinase, and Laccase. The order of enzymes activities found in this study for the isolated microorganisms is Amylase > Cellulase > Protease > Pectinase>Catalase > Gelatinase> Chitinase and Laccase. Growth medium was standardised to enhance enzyme activity. Effect of pH, and temperature on enzyme activities were tested.

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