IDENTIFICATION OF BIOLOGICALLY ACTIVE COMPOUNDS FROM Alternanthera sessilis LEAF EXTRACT ITS ANTIMICROBIAL ACTIVITY AND ANTICANCER PROPERTY

Author(s): Gopinath L. R., Kala. K., Jeevitha. S., Jeevitha. S., and Archaya, S

Abstract: Human beings consume maximum of grains followed by fruits and leaves, among this leaf occupy a unique status generally known for its vitamins minerals and therapeutic compounds. Among these therapeutic compounds recently gaining importance Alternanthera sessilis is one such leafy vegetable used on regular basis for general health maintenance. However, identification of compounds and their therapeutic activity needs their isolation which occur through dissolving in solvents. In the present study three solvents water, methanol and ethanol were used to identify the secondary metabolites though all the compounds were identified from all the three solvents methanol lead to positive result in most of the tests for secondary metabolites. Quantification of major secondary metabolites showed high saponins followed by alkaloids and tannins. GCMS analysis of methanol crude extract of A. sessillis showed 16 compounds were most of them were alkane, alcohols and esters which were known for antimicrobial and anticancer property. Methanol crude extract of A. sessillis was with K. pneumonia and B. subtilis 13.33mm followed by P. mirabilis 12.83mm and S. pneumonia 12.67mm. The DPPH scavenging activity reached up to 73.31% with 800 μg/ml and ABTS reached up to 84.29 with 800 μg/ml when methanol crude extract of A. sessillis was used. The methanol crude extract of A. sessilis showed least IC50 with HeLa 458.64 μg/ml followed by MCF 497.76 μg/ml HepG2 513.21 μg/ml cell lines.

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