IDENTIFICATION OF BIOLOGICALLY ACTIVE COMPOUNDS FROM Adhatoda vasica LEAF EXTRACT ITS ANTIMICROBIAL ACTIVITY AND ANTICANCER PROPERTY

Author(s): Gopinath L. R., Ramya Bharathi, V., Kala. K and Bhuvaneswari, R., Archaya. S

Abstract: Adhatoda vasica is one such plant which is used widely for a variety of purposes which forms part of many other traditional herbal medicines. Biologically active compounds present in the plants are responsible for such curative activity. These compounds could be identified by dissolving them in appropriate solvents. In the present study water, methanol and ethanol were used as solvents. Most of the secondary compounds were found in all the solvents but methanol gave positive result more than the other. Quantification of important solvents showed that high tannin content 65.61 μg/ml followed by saponins 19.09 μg/ml and alkaloids 12.87 μg/ml with methanol crude extract of A. vasica. GCMS analysis of methanol crude extract of A. vasica showed 21 compounds of alcohols, steroids, ester, etc. mostly having antimicrobial and antioxidant property. Antimicrobial property with well diffusion method showed effective antimicrobial activity for B. subtilis 12.17mm followed by V. cholera 11.83mm and K. pneumonia 11.50mm. Antioxidant activity was performed with DPPH assay and ABTS assay with DPPH assay 81.81% antioxidant activity was recorded at 160μg/ml and 94.84% antioxidant activity with 160μg/ml using methanol crude extract of A. vasica. Anticancer property was estimated through cytotoxicity study with cell lines showed least IC50 with HeLa 88.24 μg/ml followed by MCF 92.80 μg/ml HepG2 111.08 μg/ml cell lines using methanol crude extract of A. vasica.

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