Study on Biofilm Forming Ability of Staphylococcus Spp. Isolated from Bovine Mastitis Milk

Author(s): Khushal Patel, Palak Rahevar and Anju Kunjadiya

Abstract: Mastitis is an inflammation of mammary gland and udder tissue. One of the vital etiological agent Staphylococcus sp., mainly Staphylococcus aureus is responsible. Biofilm forming ability of such microorganism is considered as one of the vital virulence factors. This study was designed to screen the biofilm forming ability of mastitis-causing pathogens. A total of 48 Staphylococcus spp. was investigated which were isolated from mastitis infected milk samples using Baird parker and Mannitol salt differential media having drug resistivity observed from previous study. Tube assay and Microtitre plate assay were conducted for the detection of biofilm for these antibiotic-resistant strains. In tube assay detection method, 19 strong biofilm formers containing all S. aureus; 19 moderate formers containing 12 S. aureus & 7 Staphylococcus spp. and 10 weak biofilm formers containing all Staphylococcus spp. were observed at 24 hr. and after additional incubation of 12 hr leads all the isolates to strong biofilm forming category. Similarly, in microtitre plate assay out of 31 Coagulase positive and methicillin-resistant Staphylococcus aureus, 9 were moderate and 22 were strong biofilm formers, while out of studied 17 Staphylococcus spp., 9 were observed weak and 8 were moderate biofilm formers. No significance difference was observed after more incubation period i.e. 12-24 hr. in microtitre plate assay. Tube assay can be considered as the best method for preliminary detection of biofilm as it can be used to study the growth pattern of microorganism and may help in diagnosis and treatment of such infections caused by pathogens.

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