Volume 9, Issue 1, January 2019

Estimation of Keratinase from Bacillus sp, Pseudomonas sp., and Vibrio sp., isolated from Poultry Soil Biodegradation of Chicken Feather

Author(s): Nagajothi K and Subbulakshmi M *
Abstract: The Bacillus sp, Vibrio sp, and Pseudomonas sp, were isolated from poultry waste. The chicken feathers were collected from the poultry industry at Kadalaiyur. These organisms were identified by Gram’s staining, spore staining, simple staining and various microscopic observation and biochemical tests. For degradation of feathers by using microorganisms. The keratinase enzyme was estimated by using the laboratory procedure. Degradation of feather by these organisms was carried out at temperature of 37OC for 14 days and observed visually. Degradation of Bacillus sp, Vibrio sp, and Pseudomonas sp was determined of various pH and temperature. The maximum keratinase activity was obtained at pH 7&8 and temperature at of 37O C. When optimized with different carbon, and nitrogen sources are produced the medium. Degradation of Bacillus sp, Vibrio sp, and Pseudomonas sp was determined of various pH and temperature bacteria obtained from feather collection, screened for extracellular keratinolytic activity. The selected cultures such as the Bacillus sp, Vibrio sp, and Pseudomonas sp suggested strongly of bacteria that produces keratinase in the cell free culture supernatants. The best biodegradation of feather was obtained using Bacillus sp and Pseudomonas sp.
PAGES: 1240-1243  |  17 VIEWS  21 DOWNLOADS

How To Cite this Article:

Nagajothi K and Subbulakshmi M *. Estimation of Keratinase from Bacillus sp, Pseudomonas sp., and Vibrio sp., isolated from Poultry Soil Biodegradation of Chicken Feather. 2019; 9(1): 1240-1243.