Preclinical Metabolite Identification in Rat Urine

Author(s): Sai Laxmi Kesana*, Harinadha Babu Vamaraju, Jaya Prakash Dodle and P.C. Sastry

Abstract: Drug discovery and development utilizes in vitro experimental models followed by in vivo studies, to support the investigations performed on Test molecules. Performing MET ID studies on Test molecules with the utilization of HRMS gives data with certain preliminary conclusions, thereby helps in further advancement of drug candidates. Objective: The main objective of this study to check the in vivo model for the presence of metabolites observed in in vitro studies. Materials and Methods: Sprague Dawley Rat was used to administer the Test Compound. The plasma and urine samples obtained from the experimental model were analyzed for the presence of metabolites utilizing the analytical tool LC-Q-TOF Mass spectrometry. Simultaneous extraction and separation technique in combination with high resolution accurate mass spectrometric detector was used for the parent and the metabolite identification. Results and Conclusion: The simultaneous acquisition and processing gives qualitative and quantitative information about the parent and its metabolites. Three oxidative metabolites were identified and are confirmed by MS/MS and Accurate mass analysis using the analytical tool LC-Q-TOF Mass spectrometry.

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