Isolated linseed oil from Linum usitatissimum exhibited pro-coagulant property

Author(s): Sharath Kumar M. Nandish, Jayanna Kengaiah, Chethana Ramachandraiaha, Chandrammaa, Ashwini Shivaiah and Devaraja Sannaningaiah

Abstract: Current study describes isolation, characterization and the role of Linum usitatissimum oil on blood coagulation cascade. Linum usitatissimum also commonly known as flax seed. Flax seed withholds many macro and micro nutrients such as proteins, vitamins, carbohydrates and minerals. Although, flaxseed encompasses a fixed oil known as linseed oil or Flax Seed Oil (FSO) which contains unsaturated fatty acids like linoleic acid, oleic acid and linolenic acid. Linseed oil found to exhibit anti-inflammatory, anti-oxidant and anti-microbial property. Despite of its therapeutic potential, the role of linseed oil on plasma re-calcification time was not reported. Preliminary phytochemical screening was analyzed by simple test method and instrumentation method such as RP-HPLC, IR and GC-MS. Furthermore, plasma re-calcification time and direct hemolytic activity assays were performed for Flax Seed Oil (FSO) by freshly drawn human blood. FSO showed maximum amount of lipids and steroids. The obtained results from IR predicted the presence of different functional groups such as OH, CH2, C=O, C-O and cyclic ring. While, the RP-HPLC and GC-MS profiles of FSO predicted the presence of several lipids and steroids in the extract. FSO induce the clot formation process of citrated human plasma in both PRP and PPP from the control 200sec to 100sec and 220sec to 140sec respectively. Moreover, FSO did not hydrolyze RBC cells suggesting its nontoxic property.

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