Isolation and Screening of Dye Decolorizing Bacteria from Industrial Effluent

Author(s): Padma Singh and Alka Rani*

Abstract: Wastewater effluent from the various industries contain significant amount of synthetic dyes that require treatment to prevent ground water contamination. Malachite green and crystal violet are among millions of dyes which are being use in every day to day life of a human being. During the manufacturing and processing operation 20% of the resultant color enter in to the environment they are toxic and having extremely harmful consequence. The present study was an attempt to examine the potential of isolated bacteria for decolorization of malachite green and crystal violet (Triphenylmethane dyes) from the various effluent samples which were collected from the outlet of industries of adjoining area of Haridwar. About 50 bacterial isolates were isolated through the enrichment culture method out of 50 isolates 18 bacterial isolates capable of degrading the triphenylmethane dye efficiently were screened through clear zone method. The isolates showed the clear zone at the dye concentration of 100 mg⁻1 mediums (pH 7) and temperature 370C. These results signify that bacterial isolated could effectively be used in development of alternative and eco-friendly method for the decolorization of industrial effluent.

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