Volume 9, Issue 1, January 2019

Melatonin Ameliorates Intracellular Oxidative Stress and Oleic Acid Uptake in HEPA 1-6 Cells: An In Vitro Experimental Model of Nonalcoholic Fatty Liver Disease

Author(s): A.H. Vohra and D.D. Jhala*
Abstract: Non-Alcoholic fatty liver disease (NAFLD) has emerged as a major health concern, wherein circadian changes have been directly implied as one of the key causative agents. In the present study, HEPA1-6 cells treated with oleic acid (OA) have been used as a model for NAFLD in which cell viability, intracellular oxidative stress and OA uptake are investigated. OA treatment accounted for significant decrement in cell viability at 24 and 48 h treatment. However, presence of melatonin was effective in improving cell viability indices in (OA + Mel) experimental group. Intracellular oxidative stress showed prominent fluorescence in OA treated cells whereas, OA + Mel group recorded relatively weaker fluorescence. The same was confirmed by qualitative and quantitative assessment using DCFDA staining. OA treated cells were stained with Oil red O (ORO) showed visibly higher accumulation of droplets in cytoplasm but melatonin (OA + Mel group) was effective in preventing OA accumulation. These findings provide baseline evidence on melatonin mediated amelioration of OA induced lipotoxicity and NAFLD like changes in HEPA 1-6 cells.
PAGES: 1267-1273  |  27 VIEWS  28 DOWNLOADS

How To Cite this Article:

A.H. Vohra and D.D. Jhala*. Melatonin Ameliorates Intracellular Oxidative Stress and Oleic Acid Uptake in HEPA 1-6 Cells: An In Vitro Experimental Model of Nonalcoholic Fatty Liver Disease. 2019; 9(1): 1267-1273.