Protective Effect of Apigenin from Morus indica.L against Methylglyoxal Induced Oxidative DNA Damage

Author(s): Satish Anandan, Seema Siddiqi, Syeda Farha Siraj and Urooj Asna*

Abstract: Methylglyoxal is reported to induce oxidative stress and glycation. Apigenin from Morus indica has potent antiglycation effect. Hence, the objective was to examine the effect of Apigenin derived from Morus Indica on oxidative DNA damage caused by the reaction of methylglyoxal with lysine. DNA strand breakage was investigated by Gel electrophoresis to determine the effect of apigenin on calf thymus DNA incubated with methylglyoxal and lysine. In addition, the effect of methylglyoxal on morphology of Red blood corpuscles using apigenin was also characterized by scanning electron microscope by ex-vivo assay. Apigenin prevented methylglyoxal induced oxidative stress and DNA damage in the presence of copper and also protected rupture of red blood corpuscles. The oxidative stress plays a role in methylglyoxal induced cell injury and that apigenin blocks this effect by virtue of its antioxidant property.

PAGES: 173-178  |  216 VIEWS  412 DOWNLOADS