Prevalence of Methicillin Resistant Staphylococcus aureus in Community and Hospital Environments in Assam

Author(s): Manash Mazumder*, Navneet Hazarika, Mohan Ch. Kalita and Kandarpa Kr. Saikia

Abstract: Methicillin-Resistant Staphylococcus aureus (MRSA) is an important public health concern globally. Co-resistance to multiple antibiotics in MRSA renders treatment options limited. Resistance to 23 antibiotics as per CLSI guidelines, 2011 was tested in one hundred and forty samples of Staphylococcus aureus isolates collected from both out and in patients in a tertiary care centre in Assam. Bacteria was isolated form clinical samples like urine, Pus, Blood, Throat swab and Sputum. Prevalence of MRSA was 39.68% in community and 60.32% in hospital acquired infections. High frequency of isolates was MDR. Overall, high resistance to commonly prescribed antibiotics were documented ranging from Oxacillin 117 (83.57%) followed by Cefpodoxime 104 (74.29%), Kanamycin 99 (70.71%), Cefoxitin 95 (67.86%), Ceftazidime 76 (54.29%), Ertapenem 74 (52.86%) and finally Methicillin 63 (45%). Isolates were most sensitive to Vancomycin 140 (100%) followed by Imipenum 134 (95.71%), Chloramphenicol 130 (92.86%), Meropenem 128 (91.43%), Gentamucin 119 (85%), and Pipericillin/ Tazobactam 114 (81.43%). PCR detection proved useful with 100% concordance with phenotypic detection methods of S. aureus. mecAgene was found to be prevalent in 37.86% of the total isolates. Our study advocates routine uses of molecular detection techniques as a complementary tool with the conventional tools of diagnosis.

PAGES: 156-163  |  119 VIEWS  291 DOWNLOADS