Volume 9, Issue 2, April 2019
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Polyhydroxybutyrate (PHB) Production and Optimization using Staphylococcus Gallinarum and One More Soil Isolate
Author(s): Panchal H.K.
Abstract: In this present study PHB producing strains were isolated from different sites i.e. agriculture soil, oil contaminated soil, beach soil etc. Microbes were screened for PHB production by Sudan black staining and five isolates were found to produce good amount of PHB. All five strains were morphologically and biochemically characterized. Isolates were inoculated in fermentation medium for 48 hours at 37°C and pH 7. The PHB produced was extracted and quantified. Further study involved two strains S7 and S12 based on their good PHB production capacity. They were further optimized for different media parameter such as pH, carbon and nitrogen sources. For bacterial isolate S7 fructose, yeast extract and pH 7 were found optimum carbon source, nitrogen source and pH respectively whereas for bacterial isolate S12 sucrose, NH4Cl and pH 7 were found optimum carbon source, nitrogen source and pH respectively. With physical mutagenesis experiment isolate S7 gave increase in PHB production. Overall S12 was found very good PHB producer and identified as Staphylococcus gallinarum strain S533_4 based on 16SrDNA sequencing and BLAST and phylogenetic analysis.
PAGES: 52-57 | 33 VIEWS 53 DOWNLOADS
How To Cite this Article:
Panchal H.K.. Polyhydroxybutyrate (PHB) Production and Optimization using Staphylococcus Gallinarum and One More Soil Isolate. 2019; 9(2): 52-57.