Production of Amylase by Bacillus Megaterium Isolated from Estuarine Environment

Author(s): B. Sundarapandiyan*, T. Lenin and S. Jayalakshmi

Abstract: Enzymes are important biological molecules that notably speed up all the chemical reactions. They are very crucial factors for life and serve a wide range of important functions in the body. The aim of the present study was isolation, identification, optimization and purification of amylase enzyme production by using marine bacterium Bacillus megaterium. Growth parameters of B. megaterium such as pH, temperature, salinity, nutrients of carbon and nitrogen sources and incubation time were influenced the bacterial growth. The identified optimized growth parameters are followed by pH 7, temperature 35C, salinity 2%, starch and beef extract and incubation time 30hrs were found to be ideal conditions for the bacterial strain. The culture filtrate was purified by using several methods and its enzyme activity during purification considerably increased from 42U/ml/min. to 71 U/ml/min.

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