In Vitro Assessment of Antioxidant Activity, Total Phenolic and Flavonoid Content for Various Extracts of Caesalpinia pulcherrima (L.) Sw.

Author(s): Y. Anusha*, A. Niranjan Kumar, J. Kotesh Kumar, KVNS. Srinivas, A. Srivani, G. Krishna Mohan

Abstract: The leaves and stem parts of Caesalpinia pulcherrima (L.) Sw (Fabaceae) shown in many traditional medicinal uses including folklore curative for various biological disorders. The present study is focused in determination of in-vitro antioxidant activity by DPPH free radical scavenging method, Total Phenolic Content by Folin’s ciocalteu method and Total Flavonoid Content by quantitative aluminum chloride method (using Quercetin and Catechin standards) for various extracts of C. pulcherrima. From the study it was inferred that the highest antioxidant activity was found to exhibited by leaf methanolic extract (with 92. 85% inhibition rates on free radicals at 50 μg/ mL), highest phenolic content found to be in leaf Ethyl acetate extract (70.55 mg GAE / gm extract) and highest flavonoid content found to be in leaf & stem Ethyl acetate extracts (82.3 & 73.01 mg QE & CEs / gm extract).

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