Hepatoprotective Activity of Methanolic Extract of Hibiscus Plantifolius in Paracetamol Induced Hepatotoxic Rats

Author(s): Sowjanya K, Padmalatha K, D. Rama Sekhar Reddy and Basaveswara Rao M. V

Abstract: Objective: Objective of the present study was to carry out hepato protective activity of methanolic extract of Hibiscus Plantifolius belonging to family Malvaceae. Methods: The shade dried stem part of H.Plantifolius (1 kg) was powdered and extracted with methanol using soxhletion. The extract was concentrated using rotary evaporator under reduced pressure at 40°C, till free from the solvents and thereby providing crude methanol extract which was subsequently employed for further studies. Hepato protective activity was studied by using paracetamol used hepato toxicity by using 7 experimental groups like normal control, 10% DMSO, standard, test groups at a dose of 50, 150, 300 mg/kg. And also, blood was collected, and the serum was separated by centrifuging at 300 rpm for 10 min. The collected serum was used for the assay of marker enzymes. The serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) and alkaline phosphatase (ALP) and Total bilirubin and also activity were compared with standard drug. Results: Blood samples from rats are treated with methanolic extract of H.Plantifolius (50, 150, 300 mg/kg) had significant reductions in serum markers in paracetamol administered animals, indicating the effect of the extract in restoring the normal functional ability of hepatocytes. Silymarin was used as a reference drug. Conclusion: The results of the experimental study confirmed that methanolic extract of Hibiscus Plantifolius exhibits protective effects against paracetamol induced hepatotoxicity.

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