Volume 9, Issue 1, January 2019

Knocking Down Meloidogyne Incognita Genes by Plant Delivered siRNA Has Negative Pleiotropic Effects on Nematode Vigor

Author(s): D Baliyan* and R Kumar
Abstract: RNA interference (RNAi) approaches have provided novel avenues in genomic sequencing of plant parasitic nematodes (PPNs). It provides the opportunity to recognize prospective target genes and subsequently develop new resistant transgenic plants. dsRNA constructs targeting two different Meloidogyne incognita genes were evaluated to adjudge their efficiency in reducing infectivity of M.incognita in tobacco. Stable homozygous transgenic tobacco lines were engineered, i.e. RNAi-MiTP (expressing dsRNA of MiTP gene) and RNAi-MiRpn-7 (expressing dsRNA of MiRpn-7 gene). The number of knots/plant, number of females/knot, number of egg masses g-1 root wt. and number of eggs g-1 root wt. were reduced by83.6%, 76.9%, 51.6% and 59.5% in RNAi-MiTP lines and by 72.9%, 61.5%, 40.9% and 50.1% RNAi-MiRpn-7 lines respectively in comparison to the empty vector control tobacco lines. The results from this study suggest that silencing of the MiTP and MiRpn-7 genes can significantly decrease the infective potential of this plant parasitic nematode and demonstrates a promising approach for producing broadened resistance in crops against PPNs.
PAGES: 697-704  |  21 VIEWS  23 DOWNLOADS

How To Cite this Article:

D Baliyan* and R Kumar. Knocking Down Meloidogyne Incognita Genes by Plant Delivered siRNA Has Negative Pleiotropic Effects on Nematode Vigor. 2019; 9(1): 697-704.