IN-VITRO ANTIOXIDANT ACTIVITY PROFILING OF INDIGOFERA ASTRAGALINA DC. EXTRACTS ALONG WITH ESTIMATION OF ITS TOTAL PHENOLIC AND FLAVONOID CONTENT

Author(s): B. Madhavi*, A. Niranjan Kumar, KVNS. Srinivas, J. Kotesh Kumar, G. Ravi, G. Krishna Mohan

Abstract: Indigofera astragalina (Silky Indigo) a rare herbaceous weed of Leguminosae family has shown astringent, diarrhea and toothache treating properties. The present study involves the in-vitro determination of antioxidant activity by DPPH free radical scavenging method, Total Phenolic Content by Folin’s ciocalteu method and Total Flavonoid Content by quantitative aluminum chloride method (using Quercetin and Catechin standards) for various extracts of I. astragalina. From the study it was observed that the leaf methanolic extract (with 73.70%inhibition rates on free radicals at 50μg/ mL) showed highest antioxidant activity along with highest phenolic content 40.02 mg GAE / gm extract whereas stem Methanolic and leaf Ethyl acetate extracts (90.40 & 50.43 mg QE & CEs / gm extract) showed highest flavonoid content.

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