Volume 9, Issue 1, January 2019

Acacetin Inhibits Cell Proliferation, Survival, and Migration in Human Breast Cancer Cells

Author(s): Kushal Kandhari, J. P. N. Mishra and Rana P. Singh
Abstract: Acacetin is an O-methylated flavone which is extracted from various edible and medicinal plants such as Turnera diffusa (Damiana), Robinia pseudoacacia (black locust) and honey. Acacetin has been studied to possess anti-malarial, anti-inflammatory and anti-tumor efficacy. Although, its potential as an anti-tumor agent against breast cancer has not been explored in detail. In this present study, we have studied the effect of acacetin on breast cancer cells MCF-7 and MDA-MB-468. Following treatment with acacetin, the viability of breast cancer cells was measured by MTT assay which showed 41-76% decrease. The strong growth inhibitory effect of acacetin was confirmed by trypan blue assay. The inhibitory effects of acacetin were observed on migratory potential of breast cancer cells using wound-healing assay, and western blotting showed downregulation of vimentin and N-cadherin. Cell cycle arrest was also observed using flow cytometric analysis. Acacetin inhibited ERK1/2 and AKT signaling and modulated the expression of cell cycle regulators. Overall, the results suggested that acacetin strongly decreases the survival and growth of breast cancer cells and causes cell cycle arrest along with inhibiting the migratory potential and EMT in breast cancer cells.
PAGES: 443-452  |  18 VIEWS  28 DOWNLOADS

How To Cite this Article:

Kushal Kandhari, J. P. N. Mishra and Rana P. Singh. Acacetin Inhibits Cell Proliferation, Survival, and Migration in Human Breast Cancer Cells. 2019; 9(1): 443-452.