PREVALENCE OF EXTENDED SPECTRM β-LACTAMASE AND METALLO β-LACTAMASE GENES AMONG CLINICAL ISOLATES OF NONFERMENTING GRAM NEGATIVE BACTERIA

Author(s): Sarkar Apurba Kumar* Shaker Mohammad, Asef Iqbal Mohammed, Shohael Mohammad Abdullah

Abstract: Aim: In Gram negative bacteria resistant to extended spectrum β-lactam antibiotics has been emerging rapidly worldwide over the last few years and has been ascribed predominantly to the production of extended spectrum β-lactamases (ESBLs) and Metallo β-lactamases (MBLs). Non-fermenters are very popular to produce extended spectrum β-lactamase and metallo β-lactamase. This study was designed to investigate the prevalence of different extended spectrum β-lactamases and Metallo β-lactamases encoding genes and drug resistant profiles among isolated non-fermenters. Methods: In this study non-fermenters were isolated from different clinical specimens like pus, stool and urine and were identified according to biochemical characteristics and 16s rRNA gene sequencing. Isolated non-fermenters were subjected for phenotypic and genotypic identification of extended spectrum β-lactamases and Metallo β-lactamases by using combined disk diffusion test, multiplex and simplex PCR amplifications. Results: Total 94 non-fermentative bacterial pathogens were identified with isolation rate of 24.86%. Pseudomonas aeruginosa and Acinetobacter baumannii were most predominant nonfermentative bacterial pathogens. Out of 94 isolates, 5 were found positive for both phenotypically and genotypically production of extended spectrum β-lactamases and metallo β-lactamases. Pseudomonas aeruginosa and Acinetobacter baumannii were having multiple β-lactamases genes name blaNDM, blaOXA-10 and blaSHV. Conclusion: Non-fermenters like Pseudomonas aeruginosa and Acinetobacter baumannii were having multiple β-lactamases genes likeblaNDM, blaOXA-10 and blaSHV which highlight the emerging therapeutic challenge.

PAGES: 994-1001  |  28 VIEWS  38 DOWNLOADS