DETERMINING THE IN - VITRO CHOLESTEROL-REDUCING EFFICIENCY OF LACTOBACILLUS AND ENTEROCOCCUS STRAINS ISOLATEDFROM HUMAN BREAST MILK, FECES OF BREAST-FED INFANTS AND ANIMAL MILK (GOAT, COW AND BUFFALO)

Author(s): Shaik Parvinnisa, Farhath Khanum* and Chandrasekhar.N

Abstract: The present study was designed to evaluate the cholesterol-removal efficacy in-vitro of four Lactobacillus and four Enterococcus strains. All 8 isolates exhibited Bile Salt Hydrolase (BSH) activity (1.64 to 3.09 mm of precipitation zone) and cholesterol assimilation with and without bile salt (7.7% to 95.58%) but significantly higher-cholesterol assimilation was observed with bile salts (9.157% to 95.58%), Among these NB 16 and NB12 isolates manifested significantly higher cholesterol assimilation superior to other 6isolates with deoxycholic acid (95.58% and 94.85%) and cholic acid (92.17% and 88.9%) than ox bile and TDCA, and also exhibited a high cholesterol reduction ability in natural [egg yolk (NB7-34.46% to NB16-73.82%) and skimmed milk (NB7-28.22% to NB16-72.88%)] cholesterol media than synthetic cholesterol media without bile salt and cholesterol reduction potential by the 8 isolates were optimized by different cultural conditions, among these NB16 displayed an elevated cholesterol removal ability with 83.51% at1%inoculum size, 83.9% at 24 h inoculums age., 83% at pH-7, 83.17% at 37°C incubation temperature, 83.27% at 24 h incubation time and 83.51% at 70 μg/ ml cholesterol concentration these probiotic strain could be exploited as a potential biotherapeutic agent to reduce cholesterol levels and the risk of cardiovascular diseases.

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