STUDIES ON OPTIMIZATION OF L-GLUTAMINASE FROM BACILLUS ENDOPHYTICUS RN-7

Author(s): K. Nagaraju and M.Raghu Ram*

Abstract: Cancer is one of the global problems now a days, in particular leukemia is one the threatening diseases. For elimination of cancer, there is a necessity for developing an efficient drug. L-Glutaminase is an amidohydrolase enzyme, which is the choice of interest in the treatment of lymphoblastic leukemia. This study investigates the optimization and production of extra-cellular glutaminase enzyme from forest soil samples. Days of incubation, temperature, pH, supplementary carbon and nitrogen sources and metal ions on the production of L-Glutaminase was studied and accordingly optimum conditions were determined. The isolated soil bacteria Bacillus endophyticus RN-7 is a strain that produces high levels of L-Glutaminase, under optimized culture conditions, viz., on the third day of incubation at an optimum pH of 7 and temperature of 40 degree centigrade.

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