EFFECT OF GROWTH PHASE IN ENHANCING PUFA ACCUMULATION OF FEW OLEAGINOUS FRESHWATER CHLOROPHYCEAE SPECIES

Author(s): Susmita Das, Ajitabh Bora*, Hemanta K Gogoi, Sushil Manderia, Barnali Bailung, Jadab Rajkonwar and Sanjai K. Dwivedi

Abstract: Freshwater green microalgae are potential sources of vegetarian PUFAs. The fresh water bodies harbor diverse forms of microalgae, which have hitherto remained untapped. With an aim to tap these resources, few green microalgae were evaluated and compared with the different growth phase for their PUFA composition increment with a special interest in omega-3 and omega-6 fatty acid. PUFA content of seven algal species was analyzed by culturing in BG11 medium rich in nitrogen source and grow under unique growth condition established in the laboratory (unpublished). The test species accumulated maximum lipid content during stationary phase when grown in batch mode. GC-MS analysis of lipid extracted during log phase revealed presence of only Linoleic acid, while during stationary phase, other PUFAs were detected such as Docosahexaenoic acid (C22:6) and Stearidonic acid (C18:4) in Chlorococcum humicola, Arachidonic acid (C20:4) in Ankistrodesmus falcatus, Eicosadienoic acid (C20:2) in Desmodesmus opliensis, Hexadecatrienoic acid (C16:3) in Chloralla ellipsoidea and Hexadecatetraenoic acid (C16:4) in Coelastrum microporum. This study thus established a novel growth condition and particular growth phase in which PUFA accumulation enhanced in test fresh water microalgae.

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