PHYTOCHEMICAL INVESTIGATION AND ANTIOXIDANT ACTIVITIES OF METHANOL PEEL EXTRACT OF PUNICA GRANATUM L.

Author(s): C. Sivaraj*, K. Saraswathi, R. Sindhu, S. Subasree, S.T.Sangeetha, P. Arumugam

Abstract: Peels of Punica granatum L., a well-known medicinal plant, has extensively been used in ayurvedic medicines to treat various diseases. The Punica granatum is usually found growing on river banks or near dry river beds in West Bengal and South and Central India. Phenols and flavonoids content were analyzed using Folin-Ciocalteau reagent and aluminium chloride colorimetric methods. Methanol extract of peels of Punica granatum was evaluated for the antioxidant activity using DPPH˙ radical, superoxide radical (O2. -), Fe2+ chelation assay, Fe3+ reducing power and phosphomolybdenum reduction assay. The IC50 of methanol extract of peels of Punica granatum for DPPH˙ radical, superoxide radical (O2. -) and Fe2+ chelation was 181.09, 94.66 and 51.69 μg/mL concentration. The RC50 of methanol extract of peels of Punica granatum for Fe3+ reduction and phosphomolybdenum reduction was 15.00 and 16.92 μg/mL concentration respectively. The IC50 of methanol extract of peels of Punica granatum for antidiabetic activity carried out by α-amylase enzyme inhibition assay was found to be 37.18 μg/mL concentration. From the present investigation it is concluded that methanol extract of peel of Punica granatum L. was found to be good source of natural antioxidants and can be used effectively to inhibit radicals.

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