PLANT-DERIVED FOOD PRODUCTS AS DIETARY SUPPLEMENTS FOR SUSTAINABLE UTILIZATION- AN IN VITRO AND IN SILICO APPROACH

Author(s): S. Abiraami Valli and S. Uma Gowrie*

Abstract: Sustainable utilization is the use of components of biological diversity in such a way that does not lead to its long-term decline, thereby maintaining its potential to meet the needs and aspirations of present and future generations. Investigation studies related to dietary supplements from natural edible food products have been one of the significant research interests in recent years. Using several in vitro and in silico methodologies, a study was carried out in leguminous sprouts to analyze the nutrients for its recommendation as dietary supplements for all age groups. The study was carried out with two different samples- horse gram sprouts (Macrotyloma uniflorum (Lam.) Verdc.) and mixed sprouts of combination (Cicer arietinum L. (Chick pea), Macrotyloma uniflorum (Lam.) Verdc. (horse gram) and Vigna radiata (L.) R. Wilczek (Green gram). Screening the phytoconstituents by qualitative phytochemical tests and quantification of the primary and secondary constituents were carried out in methanol and aqueous extracts of fresh and dried sprouts. Phytochemical characterization was carried out through FTIR and GC-MS analysis which proved the presence of essential phytoconstituents like terpenoids, fatty acids, proteins, carbohydrates and vitamins. Antibacterial activity of both the samples were studied against human pathogens namely Staphylococcus aureus, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and Shigella flexneri. Maximum zone of inhibition were shown by Shigella flexneri in horse gram and in mixed sprouts. Further, bioactive compounds obtained from GC-MS analysis were screened for anti-inflammatory activity and the potent bioactive compound- its structure and functions were analyzed through in silico studies using different bioinformatics tools and softwares which indicated the effective physico-chemical properties of the bioactive compounds which might be responsible for the anti-inflammatory (anti-ulcer) property. Thus, the horse gram sprouts and mixed sprouts enriched with the phytoconstituents can be recommended as a natural source of dietary supplements which can be one of the most economical and easily available source for consumption leading to sustainable utilization.

PAGES: 416-425  |  91 VIEWS  241 DOWNLOADS