EVALUATION OF HEPATOPROTECTIVE EFFECT OF INDIGOFERA TIRUNELVELICA SANJAPPA AGAINST CCl4 INDUCED HEPATOTOXICITY IN RATS

Author(s): S. Subburayalu*, KRT. Asha and A. Palavesam

Abstract: Objective: The present investigations were undertaken to evaluate the hepatoprotective of the ethanolic extract of Indigofera tirunelvelica Sanjappa (Et-It) against CCl4-induced hepatotoxicity in rats. Methods: Hepatoprotective effect of ethanolic extract of Indigofera tirunelvelica (EtL-It) was determined by using carbon tetrachloride (CCl4) intoxication of rats as experimental models. The range of liver impairment and effect of the extract of medicinal plant was evaluated by assorted biochemical markers like aspartate aminotransferase (AST), alkaline phosphatase (ALP), alanine aminotransferase (ALT), total protein (TP) and total billrubin (TB) in blood serum and concentration of Lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), glutathione-s-transferase (GST) and glutathione peroxidase (GPx), in liver were determined. Histopathological changes in the liver of different groups were also studied. Results: The administration of Et-It at dose levels of 100, 200 and 400 mg/kg/b.w., orally had decreased the rise of ALT, AST, ALP, TB and TBRAS levels and the effects were comparable to standard drug (Silymarin 20 mg/kg/b. w,) the GSH, SOD, CAT, GPx, GST and TP levels were significantly increased in the animals received EtL-It. The histopathological studies show decreased necrosis and hepatocellular degeneration when compared to the CCl4 intoxicated liver. Conclusion: This study demonstrates that the hepatoprotective of Indigofera tirunelvelica therefore scientifically supports the use of this plant in traditional medicine for treatment of liver disorders.

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