IN VITRO CALLUS INDUCTION AND SHOOT REGENERATION OF DECALEPIS ARAYALPATHRA (JOSEPH AND CHANDRAS) VENTER

Author(s): *Raveesha H R and Ashalatha K S

Abstract: The aim of the present study was to develop an efficient protocol for callus induction, shoot regeneration, biomass and antioxidant activity of Decalepis arayalpathra. The different explants were cultured on MS medium supplemented with different concentration of auxins and cytokinins. The total phenolic content and antioxidant activities were determined by standard procedures. Callus induction was higher from leaf explants on media supplemented with individual growth regulator (2 mg/L of 2, 4-D, and 1 mg/L of BAP). However, combination of BAP (2 mg/L) + 2, 4-D (1 mg/L) and BAP (1 mg/L) + NAA (0.5 mg/L) resulted in maximum callus induction. The callus biomass was significantly higher in medium supplemented with BAP (2 mg/L) + NAA (1 mg/L). Shoot regeneration was higher from shoot tip and nodal explants on medium fortified with BAP (1.0 mg/L) + NAA (0.2 mg/L) & IBA (0.5 mg/L). Root induction was maximum on half strength MS medium supplemented with IBA (0.5 mg/L) and NAA (0.2 mg/L). The plants were subjected to hardening and showed around 70% of survival after acclimatization. The total phenolic content and antioxidant activities were significantly higher in methanolic extract of leaf compared to callus. Our results demonstrated that leaf and nodal explants are the good source for the callus induction and shoot regeneration, which might be helpful in mass propagation and conservation of this medicinally important plant.

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