PLASMID PROFILING OF SALMONELLA TYPHIMURIUM ISOLATES FROM COMMERCIAL POULTRY FARMS OF HARYANA

Author(s): Vipin Khasa*, Pamela Singh and Parveen Kaur Sidhu

Abstract: Non-typhoidal salmonellosis is major cause of diarrhea in human and animals throughout the world leading to huge economic loss and mortality. Human Salmonellosis is sometimes traced back to animal source, majority of them from poultry and its products. Keeping this in mind, eleven strains of Salmonella typhimurium were isolated from different districts of Haryana; from broiler affected with Salmonellosis, which were revived and plasmid extraction was performed for profiling. The strains were classified into three plasmid types based on number and size of plasmids. Antibiotic sensitivity tests were also performed on all isolates and compared with plasmid. All plasmid types had 90kb plasmid whereas additional plasmid of size 110kb, 120kb and 23 kb were also observed in only two plasmid types. Plasmid type-1 and 2 had no major difference when checked for antibiotic resistance whereas plasmid type-3, having large size plasmids (110 and 120 Kbp) were found to exhibit resistance against the twelve antibiotics out of sixteen antibiotics used. Hence, high molecular weight plasmids may have role in antibiotic resistance and whereas plasmid between 50-100 kps may be serovar specific virulence genes.

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