PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF NATURAL DYE FROM BETA VULGARIS L.

Author(s): Treesa Santhosh and T.Vaithiyanathan*

Abstract: Betalain pigment of Beta vulgaris (L.) comprises an excellent natural dye. Beta vulgaris (L.) is good source of much health promoting and protective phytochemicals. The present study was conducted to assess the qualitative phytochemical analysis and in-vitro antibacterial activities of the Beta vulgaris natural dye. Methods: The Beta vulgaris (L.) natural dye was screened to determine the presence of alkaloids, carbohydrates, glycosides, phytosterols, tannins, phenolic compounds, flavonoids, terpenoids, saponins and quinones through preliminary phytochemical screening. And bacterial strains were subjected to antibiotic sensitivity testing by Kirby–Bauer’s disc diffusion method. The antibacterial activity was calculated based on the zone of inhibition and activity index using Muller–Hinton broth in a spread plate method. Results: The dye extract of Beta vulgaris (L.) revealed the presence of carbohydrates, glycosides, phytosterols, phenolic compounds, tannins, flavonoids, terpenoids, saponins and quinones. The antibacterial analysis showed that Beta vulgaris dye extract does not allow the growth of bacteria due to its antibacterial activity. Natural dye of Beta vulgaris (L.) possessed highest antibacterial activity against Staphylococcus aureus. Conclusions: Beta vulgaris natural dye has the potential to be developed as antibacterial agents against some bacteria due to the presence of active phtyochemicals.

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