IN VITRO STUDIES ON ALPHA AMYLASE INHIBITORY ACTIVITY OF INDIAN MEDICINAL PLANTS

Author(s): *Hemlata Bhosale, Pooja Sonsale and Uzma Shaheen

Abstract: Pancreatic alpha amylase inhibitory activity of aqueous extracts of medicinal plants was evaluated in vitro to search new anti-diabetic agents as alternatives to synthetic medicines. Rhizome and leaves of C. longa and leaves of Moringa oleifera L., Azadirachta indica L., Psidium guajava L. and Murraya koenigii L were extracted with hot water and six extracts were tested for presence of PPA inhibitory activity quantitatively and their modes of inhibition were determined. Presence of alpha amylase inhibitors were identified in all extracts in quantitative assay. Aqueous extract of leaves and rhizome of C. longa showed highest anti-amylase potential with IC50 values of 0.53±0.10 and 0.96±0.29 mg/ml respectively. IC50 values of other extracts ranged between 1.24±0.49 to 4.50±0.38 mg/ml. Three highest inhibition potential showing extracts of C. longa leaves and rhizome and aqueous extract of Moringa oleifera displayed non-competitive, mixed and non-competitive mode of inhibition respectively as determined in terms of changes in Vmax and km values. In conclusion, active constituents of these three extracts possess anti-diabetic properties and can be used in management of diabetes mediated complications.

PAGES: 636-640  |  35 VIEWS  59 DOWNLOADS